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Bacterial Transformation: Students observe the link between genotype and phenotype by genetically engineering bacteria with genes for antibiotic resistance and fluorescence. Following overnight incubation, transformed bacteria are compared with controls for the ability to express the expected protein products.
Protein Purification: Purification of natural and bacterial expressed recombinant protein is part of our everyday lives. Students explore these methods through a stepwise purification of GFP from bacteria.
DNA Restriction: The ability to reliably and predictably cut DNA according to its sequence has revolutionized molecular biology. In this course, students recreate the Nobel prize winning experiment of cutting l DNA with restriction enzymes and use gel electrophoresis to separate and visualize the resulting DNA fragments.
DNA Restriction and Ligation: Building on DNA Restriction, this course illustrates the complementary roles of restriction enzymes and ligase. Students cut plasmid DNA and then ligate the resulting fragments back together. In using gel electrophoresis to visualize the results, students are given a first introduction to complexities of cloning technique.
DNA Fingerprinting (4 hrs): This lab explores DNA polymorphisms, which are the basis of forensic DNA fingerprinting and genetic diagnosis. Students prepare a sample of their own DNA from cheek cells and use polymerase chain reaction (PCR) to amplify a polymorphic region of chromosome 16. Results are analyzed by agarose gel electrophoresis.
DNA Barcoding – grades 6-12 – 3 days: 4hrs; 2.5hrs; 2.5hrs: This multi-session course explores how DNA sequence data has revolutionized modern taxomony. By “DNA Barcoding” specimens from the world around them, students use DNA sequence to document and classify living organisms.
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