The difference in the diversity of gut microbes in healthy and ADHD-positive zebrafish(Danio rerio)
Project:
The difference in the diversity of gut microbes in healthy and ADHD-positive zebrafish(Danio rerio)
Students:
Sirui Qian, Ziyu Xie, Tsanzi Wang
School:
Xing Hai school; Ulink college of Shanghai; Dulwich College Suzhou, Suzhou, China
Mentors:
Xinyue Wang
Abstract:
Since the 1960s, the zebrafish (Danio rerio) has become increasingly frequent in scientific research, one reason being that their genetic makeup is very similar to humans. Research in recent years has confirmed a link between gut microbe and attention deficit hyperactivity disorder (ADHD) in humans. For this project, we aim to study the difference in the diversity of of gut microbe in healthy zebrafish and ADHD-positive zebrafish. We will be using DNA barcoding to extract and amplify the CO1 barcode region to confirm the species of the zebrafish. Then, we will use the Qiagen Powersoil Pro Kit to extract bacterial genomic DNA from gut samples of healthy zebrafish and ADHD-positive zebrafish. We will then use Oxford Nanopore Technologies to amplify bacterial 16S and sequence barcode libraries. We expect our research results to indicate similar outcomes as the previous studies that have been done on humans, which in turn can further validate the zebrafish as a relevant model for scientifi
Poster:
View team poster (PDF/PowerPoint)
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